MATERIAL:
- Filter paper
- Spinachs
- Petri dish
- Ethanol
- A mortar
- Calcium carbonate
- A spatula
PROCEDURE:
- First of all we have to wash the spinach to clean it and then we dry it with a paper.
- Then we have to put the washed spinach in the mortar and grind it until we get a green liquid.
- Then we have to add 50mL of ethanol and a very little bit of calcium carbonate with the spatula.
- We romove it and then we have to filter the mixture with a filter paper.
- Put a part of the mixture in the petri dish and put the paper, that we have folded in a half.
- We have to wait until we can observe the different pigments.
RESULTS:
In our cromatography we could saw the following pigments:
- The orange or red pigment is the beta carotene
- The dark green is the alfa chlorophyll
- The light green is the beta chlorohyll
- The yellow pigment is the xanthophylls
QUESTIONS:
- Why do we add calcium carbonate? Because thanks to add calcium carbonate we don't degrade the pigments.
- Which is the colous of every pigment? Is in the results.
- For what purpose it does have different colour pigments? To know the solubility of different pigments.
- Why do they separate in the paper?
