Catalasa

OBJECTIVE: Know the functions of catalasa and the quantity
MATERIAL:

• Liver's pieces
• Potato's pieces
• H₂O₂
• Two test tubes
• Four beakers
• Two thermometers
• Knife
• Wire gauze
• Bunsen burner

PROCEDURE:

1. We cut the liver and the potato into pieces. We have to add some H₂O_2. Then we put some pieces of each material in two different beakers. Observe what is happening
2.                      
3. We have to repeat the same but in a closed container. We use a test tube with a cork, this cork has a hole to put a thermometer in it. We write the temperature variation every 30 seconds, during 3 minutes.                                                         
4. We put some pieces of liver and potato together in a beaker with hydrogen peroxide and we boil it using a wire gauze and a bunsen burner. We will see that hydrogen peroxide doesn't poduce bubbles, that happens because catalasa enzymes are not reacting. We have denatured the enzyme and thats because catalasa doesn't react.

                                                  
RESULTS:

As we can see in the board, the liver's temperature goes up and the potato's doesn't.