Last week we went to the PRBB to do different experoments. In my case I do the electroforesy with the genes of a worms called C-elegance.
The electroforesy is used to know the large of this genes.
MATERIAL:
- Gloves
- Pipette
- 4 samples of different gens: daf-18 mutat, daf-18 silenciat, GFP+RFP -> fluorescència i Salvatge
- The electrophoresis
PROCEDURE:
- Put the genes with a pipette the different tipes of genes in a gel in a wells that are in the gel
- Then cover the gel and electrify.
- These genes are going to move, ones more than others, depending of the largest of every tipe of genes.
RESULTS:
|
MICROSCOPIA
|
IMATGE
|
GENÉTICA
|
COMPORTAMENT
|
RESULTATS
|
A
|
male:
hermafrodita:
|
I:
F
|
short
|
+sal
-,-,+--> Si
|
DAF-18 mutat
|
B
|
male:
hermafrodita: |
I:
F |
large
|
+sal
-,-,+--> Si
|
GFP+RFP
|
C
|
male:
hermafrodita: |
I:
F |
large
|
+sal
-,-,+--> Si
|
SALVATGE
|
D
|
male:
hermafrodita: |
I:
F |
large
|
+,+,+,--> No
|
DAF-18 silenciat o apagat
|