PRBB ( Parc de Recerca Biomèdica de Barcelona)

Last week we went to the PRBB to do different experoments. In my case I do the electroforesy with the genes of a worms called C-elegance.

The electroforesy is used to know the large of this genes.

MATERIAL:

• Gloves
• Pipette
• 4 samples of different gens: daf-18 mutat, daf-18 silenciat, GFP+RFP -> fluorescència i Salvatge
• The electrophoresis

  
                          
PROCEDURE:

1. Put the genes with a pipette the different tipes of genes in a gel in a wells that are in the gel
2. Then cover the gel and electrify. 
3. These genes are going to move, ones more than others, depending of the largest of every tipe of genes.

  

RESULTS:

	MICROSCOPIA	IMATGE	GENÉTICA	COMPORTAMENT	RESULTATS
A	male: hermafrodita:	I: F	short	+sal -,-,+--> Si	DAF-18 mutat
B	male: hermafrodita:	I: F	large	+sal -,-,+-->  Si	GFP+RFP
C	male: hermafrodita:	I: F	large	+sal -,-,+-->  Si	SALVATGE
D	male: hermafrodita:	I: F	large	+,+,+,--> No	DAF-18 silenciat o apagat