The objective is to see the different phases of the cell mitosis with a rood of an onion.
MATERIAL:
- Beaker
- Slide and cover slide
- Forceps
- Watch glass
- Filter paper
- Lighter
- Bunser burner
- Orcein A
- Orcein B
- Onion
- Scissors
- Dropper
- Microscope
PROCEDURE:
- Cut a root of the onion and put it in a watch glass.
- Add 2ml of Orcein A to the root.
- Open the bunsen burner and warm it up until we see weak vapors.
- Then take the root with the forcepsand put it on a slide and add some drops of Orcein B.
- Put the slide on the filter paper and put the cover slide. With the paper filter wrap the slide and clean out the excess of Orcein B.
- Put the slide in the microscope and observe it.
RESULTS:
When we watch the sample on the microscope we have to see the phases of mitosis if we have done it well.
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